Journal: Hypertension

Article Title: Dipeptidyl Peptidase IV Regulates Proliferation of Preglomerular Vascular Smooth Muscle and Mesangial Cells

doi: 10.1161/hypertensionaha.112.196501

Figure Lengend Snippet: Figure 1. A, Dipeptidyl peptidase IV (DPPIV) mRNA levels in spontaneously hypertensive rat (SHR) and Wistar-Kyoto rat (WKY) preglomerular microvascular smooth muscle cells (PGVSMCs) and glomerular mesangial cells (GMCs). DPPIV mRNA expression was determined by quantitative real-time PCR and normalized to -actin mRNA. B, Western blotting for DPPIV protein using 2 different antibodies (Ab 1 and 2). A dis- tinct and single band was observed at 55 kDa for both WKY and SHR PGVSMCs and GMCs. C, DPPIV protein expression in SHR and WKY PGVSMCs and GMCs. DPPIV protein expression was quantified by densitometry analysis of Western blots and normalized to -actin protein. Letter above bars (a) indicates significantly different (Fisher least significant difference [LSD] test) from PGVSMCs.

Article Snippet: To ensure that the signal detected by Western blotting was indeed DPPIV, we performed Western blotting for DPPIV protein as described in Jackson et al.3 using two different primary antibodies: 1) rabbit polyclonal anti-DPPIV antibody (Proteintech Group, Inc.; Chicago, IL; catalog No. 10940-1-AP; 1:600); and 2) rabbit polyclonal anti-DPPIV antibody (Thermo Scientific, Rockford, IL; catalogue number PA1-8455; 1:2500).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot

Journal: Frontiers in Cell and Developmental Biology

Article Title: Adiponectin receptor T-cadherin emerges as a novel regulator of adipose stem cell quiescence and adipogenesis

doi: 10.3389/fcell.2025.1734183

Figure Lengend Snippet: Double immunofluorescent staining of human subcutaneous adipose tissue sections with antibodies against T-cadherin (green) and DPP4 (red); nuclei were counterstained with DAPI (blue). Images were acquired using a Zeiss LSM 780 confocal microscope and ZEN2010 software, shown at lower magnification (A) and higher magnification (B) . A thick arrow points to a group of cells expressing both T-cadherin and DPP4 in the interstitium; thin arrows mark cells expressing only T-cadherin; ovals encircle adipocytes. Scale bar 50 µm. (C) The table shows the percentage of T-cadherin–positive, DPP4 + cells and double-positive cells (DPP4 + /T-cadherin + ), quantified from adipose tissue sections of two healthy donors.

Article Snippet: Cells were detached from culture dishes using HyQTase Detachment Reagent (HyClone, GE Healthcare Life Sciences, United States) and stained with appropriate combinations of primary antibodies against: DPP4 (CD26 Antibody (MA2607), ThermoFisher Scientific, dilution 1:100), T-cadherin (ProSci, United States, #3583, dilution 1:100).

Techniques: Staining, Microscopy, Software, Expressing

Journal: Frontiers in Cell and Developmental Biology

Article Title: Adiponectin receptor T-cadherin emerges as a novel regulator of adipose stem cell quiescence and adipogenesis

doi: 10.3389/fcell.2025.1734183

Figure Lengend Snippet: Light microscopy of MSCs (of the two to three passages) isolated from human subcutaneous adipose tissue of a healthy donor (A) and immunofluorescent staining with antibodies against T-cadherin (green) (B) . Arrows indicate cells with low or no T-cadherin expression, whereas cells exhibiting green fluorescence corresponding to T-cadherin are clearly visible. Scale bar, 50 µm. Light microscopy of human MSCs (C) and double immunofluorescent staining with antibodies against T-cadherin green, (E) and DPP4 red, (F) nuclei were counterstained with DAPI blue, (D) . Arrows in (C–F) indicate one and the same cell co-expressing T-cadherin and DPP4. Images were acquired using a Leica DMI 6000B microscope equipped with a Leica DFC7000T digital camera and LAS X software. Scale bar, 20 µm. (G) Representative flow cytometry plot showing T-cadherin and DPP4 distribution in cultured MSCs. The proportion of double-positive (DPP4 + /T-cadherin + ) cells was 30.4%; 6.15% expressed only T-cadherin, and 14% expressed only DPP4.

Article Snippet: Cells were detached from culture dishes using HyQTase Detachment Reagent (HyClone, GE Healthcare Life Sciences, United States) and stained with appropriate combinations of primary antibodies against: DPP4 (CD26 Antibody (MA2607), ThermoFisher Scientific, dilution 1:100), T-cadherin (ProSci, United States, #3583, dilution 1:100).

Techniques: Light Microscopy, Isolation, Staining, Expressing, Fluorescence, Microscopy, Software, Flow Cytometry, Cell Culture

Journal: Frontiers in Cell and Developmental Biology

Article Title: Adiponectin receptor T-cadherin emerges as a novel regulator of adipose stem cell quiescence and adipogenesis

doi: 10.3389/fcell.2025.1734183

Figure Lengend Snippet: Individual UMAP plots showing the expression levels and distribution of CDH13 (encoding T-cadherin) in control MSCs (A) and MSCs after 4 days of adipogenic induction (B) . UMAP plots demonstrating DPP4 expression in control MSCs (C) and MSCs after 4 days of adipogenic induction (D) . (E) RT-qPCR analysis of MSCs cultured in control medium or under adipogenic induction conditions showing the dynamics of T-cadherin mRNA expression. T-cadherin/ CDH13 expression decreased by day 4 in adipogenic medium and remained low through day 10. RT-qPCR data are shown as the mean ± SD. T-test. **р< 0.01 *p < 0.05 vs. control media in corresponding experimental day. Results are representative of three biologically independent experiments.

Article Snippet: Cells were detached from culture dishes using HyQTase Detachment Reagent (HyClone, GE Healthcare Life Sciences, United States) and stained with appropriate combinations of primary antibodies against: DPP4 (CD26 Antibody (MA2607), ThermoFisher Scientific, dilution 1:100), T-cadherin (ProSci, United States, #3583, dilution 1:100).

Techniques: Expressing, Control, Quantitative RT-PCR, Cell Culture

Journal: Frontiers in Cell and Developmental Biology

Article Title: Adiponectin receptor T-cadherin emerges as a novel regulator of adipose stem cell quiescence and adipogenesis

doi: 10.3389/fcell.2025.1734183

Figure Lengend Snippet: Integrated object. (A) FeaturePlot–UMAP-plot showing principal distribution of CDH13 gene expression (encoding for T-cadherin) in the integrated object; CDH13 expressing cells corresponds to Cluster 3 (more than 1-fold change of the average expression level); (B) FeaturePlot–UMAP-plot showing principal distribution of DPP4 gene expression (encoding for T-cadherin) in the integrated object; DPP4 expressing cells correspond to Cluster 3 (more than 1-fold change of the average expression level) (C) DimPlot–Integrated object UMAP-clustering. Sample proportion diagrams depict the ratio between the cell counts in the control MSC sample (Salmon) and in the MSC sample (Iris blue) after a 4-day induction of adipogenic differentiation within the Clusters. (D) DimPlot–Integrated object grouped by samples. CDH13 expression in the control MSC sample (Salmon) and MSC sample (Iris blue) after a 4-day induction of adipogenic differentiation. Cluster 3 predominantly contains cells from the control sample.

Article Snippet: Cells were detached from culture dishes using HyQTase Detachment Reagent (HyClone, GE Healthcare Life Sciences, United States) and stained with appropriate combinations of primary antibodies against: DPP4 (CD26 Antibody (MA2607), ThermoFisher Scientific, dilution 1:100), T-cadherin (ProSci, United States, #3583, dilution 1:100).

Techniques: Gene Expression, Expressing, Control

Journal: Frontiers in Cell and Developmental Biology

Article Title: Adiponectin receptor T-cadherin emerges as a novel regulator of adipose stem cell quiescence and adipogenesis

doi: 10.3389/fcell.2025.1734183

Figure Lengend Snippet: Integrated object. FeaturePlot. Each cluster is denoted by color. Cluster 0 (Salmon) primarily contains cells expressing fibroblast markers and genes responsible for cell cycle regulation. Cluster 1 (Khaki) encompasses cells expressing preadipocyte-specific genes, such as CEBPB , PPARγ, CD36 and markers of mature adipocytes ( ADIPOQ , Perilipin1 , Perilipin4 ). In Cluster 2 (green), cells predominantly express genes related to mitosis. Cluster 3 (Blue) contains cells of interest with high level of T-cadherin expression, as well as classical MSC markers ( CD90 , PDGFR ), Wnt signaling genes , and DPP4 . In a separate remote Cluster 4 (Magenta), besides CDH13 , cells express Nestin , a marker of neural crest cells, and CD36 , a marker of adipocyte progenitors.

Article Snippet: Cells were detached from culture dishes using HyQTase Detachment Reagent (HyClone, GE Healthcare Life Sciences, United States) and stained with appropriate combinations of primary antibodies against: DPP4 (CD26 Antibody (MA2607), ThermoFisher Scientific, dilution 1:100), T-cadherin (ProSci, United States, #3583, dilution 1:100).

Techniques: Expressing, Marker

Journal: Frontiers in Cell and Developmental Biology

Article Title: Adiponectin receptor T-cadherin emerges as a novel regulator of adipose stem cell quiescence and adipogenesis

doi: 10.3389/fcell.2025.1734183

Figure Lengend Snippet: Split violin-plots showing the relative expression levels and distribution of CDH13 (A) and DPP4 (B) genes in the control MSC sample (Salmon) and MSC sample after a 4-day induction of adipogenic differentiation (Iris blue). The highest CDH13 expression was detected in Cluster 3 in MSCs of the control sample compared to MSCs after a 4-day adipogenic induction. Similarly, the highest expression of DPP4 was found in Cluster 3 in MSCs of the control sample. Split violin plots were generated using the R package Seurat and the function VlnPlot with the argument split.by = “sample”.

Article Snippet: Cells were detached from culture dishes using HyQTase Detachment Reagent (HyClone, GE Healthcare Life Sciences, United States) and stained with appropriate combinations of primary antibodies against: DPP4 (CD26 Antibody (MA2607), ThermoFisher Scientific, dilution 1:100), T-cadherin (ProSci, United States, #3583, dilution 1:100).

Techniques: Expressing, Control, Generated

Journal: Frontiers in Cell and Developmental Biology

Article Title: Adiponectin receptor T-cadherin emerges as a novel regulator of adipose stem cell quiescence and adipogenesis

doi: 10.3389/fcell.2025.1734183

Figure Lengend Snippet: (A) DimPlot– GSE182158 object UMAP-clustering; (B) 2 cluster manual cell type annotation, the red oval marks cluster 2; (C) FeaturePlot–UMAP-plot showing principal distribution of CDH13 gene expression in the GSE182158 object; (D) FeaturePlot–UMAP-plot showing principal distribution of DPP4 gene expression (encoding for T-cadherin) in the GSE182158 object.

Article Snippet: Cells were detached from culture dishes using HyQTase Detachment Reagent (HyClone, GE Healthcare Life Sciences, United States) and stained with appropriate combinations of primary antibodies against: DPP4 (CD26 Antibody (MA2607), ThermoFisher Scientific, dilution 1:100), T-cadherin (ProSci, United States, #3583, dilution 1:100).

Techniques: Gene Expression

Journal: Frontiers in Cell and Developmental Biology

Article Title: Adiponectin receptor T-cadherin emerges as a novel regulator of adipose stem cell quiescence and adipogenesis

doi: 10.3389/fcell.2025.1734183

Figure Lengend Snippet: Elevated DPP4 expression in MSCs after lentiviral transduction in T-cadherin-overexpressing cells was verified using RT-qPCR (A) and Western blot (B) . β-tubulin was used as the loading control for Western blot analysis. Representative results from one of two biologically independent RT-qPCR and eight Western blot experiments are shown. ANOVA with multiple comparisons, **p < 0.01.

Article Snippet: Cells were detached from culture dishes using HyQTase Detachment Reagent (HyClone, GE Healthcare Life Sciences, United States) and stained with appropriate combinations of primary antibodies against: DPP4 (CD26 Antibody (MA2607), ThermoFisher Scientific, dilution 1:100), T-cadherin (ProSci, United States, #3583, dilution 1:100).

Techniques: Expressing, Transduction, Quantitative RT-PCR, Western Blot, Control

Journal: Drug Metabolism and Disposition

Article Title: Wuzhi Tablet ( Schisandra Sphenanthera Extract) Protects against Acetaminophen-Induced Hepatotoxicity by Inhibition of CYP-Mediated Bioactivation and Regulation of NRF2-ARE and p53/p21 Pathways

doi: 10.1124/dmd.114.059535

Figure Lengend Snippet: WZ possesses marked inhibitory effects on the activities of P450 enzymes and NAPQI-GSH formation. (A) Western blot analysis of CYP2E1, CYP1A2, CYP3A11, and glyceraldehyde-3-phosphate dehydrogenase levels in livers from control, WZ (700 mg/kg)-treated, APAP-treated, WZ (700 mg/kg)/APAP-treated mice. (B) Densitometric analysis of Western blots (n = 3). (C) Effect of WZ on the activities of CYP2E1, CYP1A2, CYP3A11 enzymes in mouse liver microsomes (n = 5). (D) Effect of WZ on NAPQI-GSH formation in mouse liver microsomes (n = 5). *P < 0.05, **P < 0.01, ***P < 0.001 versus control mice; #P < 0.05, ##P < 0.01 versus APAP-treated mice.

Article Snippet: CYP1A2 and CYP2E1 antibodies were purchased from Boster Biotechnology Co., Ltd. (Wuhang, China).

Techniques: Western Blot, Control

Journal: BMC Cancer

Article Title: Serum CD26 levels in patients with gastric cancer: a novel potential diagnostic marker

doi: 10.1186/s12885-015-1757-0

Figure Lengend Snippet: CD26 serum levels in patients with gastric cancer ( n = 30) and in control healthy individuals ( n = 24). Patients serum CD26 levels: 557.7 ± 118.3 pg/mL; control serum CD26 levels: 703.4 ± 170.3 pg/mL. * p = 0.001 by unpaired t test

Article Snippet: The concentration of serum CD26 were analyzed using a specific immunoassays (Human CD26/DPP4 ELISA Kit, Boster biological technology, Pleasanton, CA) ELISAs were performed according to the manufacturer’s instructions: mean values of duplicated measurements were calculated and a sigmoid-shaped standard curve was determined by simultaneously analyzing a dilution series of standard samples.

Techniques: Control

Journal: Pharmaceutical Biology

Article Title: Ethanol extract of the mushroom Coprinus comatus exhibits antidiabetic and antioxidant activities in streptozotocin-induced diabetic rats

doi: 10.1080/13880209.2022.2074054

Figure Lengend Snippet: Dipeptidyl peptidase (DPP)-4 levels after extract treatment. Histograms with the same letter are not significantly different, values are expressed as mean ± SD ( n : 24) p < 0.05. HC: Healthy control; NC: negative control (45 mg STZ); PC: positive control (45 mg metformin); T1: 250 mg ethanol extract of C. comatus ; T2: 500 mg ethanol extract of C. comatus ; T3: 750 mg ethanol extract of C. comatus .

Article Snippet: Rat-specific DPP-4 (Cat.No E0226Ra), insulin (Cat.No E0707Ra), and GLP-1 (Cat.No E0719Ra) ELISA kits (BT Laboratories, Shanghai, China) were used for the measurement of the corresponding parameters (BT Laboratory ).

Techniques: Control, Negative Control, Positive Control